Journal: iScience

Article Title: Human iPSC cardiomyocyte patch transplantation modifies extracellular matrix and fibroblast behavior after myocardial infarction

doi: 10.1016/j.isci.2026.115341

Figure Lengend Snippet: TGF-β secreted from the hiPSC-derived CM patch induced Col1a1 expression but not Col3a1 (A) Schematic representation of the co-culture system. hiPSC-derived CMs were seeded on the upper chamber, whereas the cardiac fibroblasts isolated from mice heart were cultured at the lower chamber in the presence and absence of SB431542, a TGF-β receptor inhibitor. (B) RT-qPCR analysis of Col1a1 , Col3a1 , and Pai1 in the cultured cardiac fibroblasts. Two-way ANOVA (co-culture × inhibitor) with interaction; Tukey-adjusted post hoc tests on estimated marginal means. Data represent mean ± SEM. N = 3–6, one-way ANOVA followed by Tukey’s HSD test, p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: Then proteins were analyzed using primary antibodies for Col1a1 (R1038, OriGene, Rockville, MD), Col3a1(ab6310, Abcam, Cambridge, UK) and Gapdh (AM4300, Thermo Fisher Scienctific, Waltham, MA), respectively on Amersham Imager 600 (GE Healthcare, Chicago, IL).

Techniques: Derivative Assay, Expressing, Co-Culture Assay, Isolation, Cell Culture, Quantitative RT-PCR

Journal: Future Science OA

Article Title: The potential of plant-derived exosome-like nanovesicles from Centella asiatica leaves (CA-PDENs) for anti-inflammation and prevention of UVB-induced photoaging

doi: 10.1080/20565623.2026.2654777

Figure Lengend Snippet: Results of antioxidant and pro-collagen effects of CA-PDENs. (A) Graphical depiction of DPPH radical scavenging activity of CA-PDENs and vitamin C within the concentration of 6.25-400 µg/mL, data are presented as mean ± standard deviation (n = 3). (B) Fluorescence intensity in UVB-induced 1BR3 cells represents the level of intracellular ROS after CA-PDENs treatment for 24 hours; data are presented as mean ± standard deviation (n = 3). *p < 0.05, **p-value < 0.01 vs. negative control. (C) Confocal microscopy images showing collagen type I expression in 1BR3 cells after 6 hours of CA-PDENs incubation at the concentrations of 40 and 100 µg/mL (200× magnification). Nuclei were stained with DAPI (blue), while collagen type 1 was labeled with Alexa Fluor 647 (red). Merged images show the localization of collagen type 1 expression in the cytoplasmic region surrounding the nuclei. (D) Collagen type I levels in UVB-induced 1BR3 cells followed by CA-PDENs incubation at 40 and 100 µg/mL for 48 hours, data are presented as mean ± standard deviation (n = 3). **p-value < 0.01 vs. control. Abbreviations: CA-PDENs, Centella asiatica -derived exosome-like nanovesicles; DPPH, 2,2-diphenyl-1-picrylhydrazyl; ROS, reactive oxygen species; UVB, ultraviolet B; DAPI, 4′,6-diamidino-2-phenylindole.

Article Snippet: Cells were incubated overnight at 4 °C with a mouse anti-collagen type I primary antibody (1:500 in 1% BSA; Invitrogen, USA), followed by incubation with an Alexa Fluor 647-conjugated goat anti-mouse IgG secondary antibody (1:500 in 1% BSA; Abcam, USA) for 60 minutes in the dark.

Techniques: Activity Assay, Concentration Assay, Standard Deviation, Fluorescence, Negative Control, Confocal Microscopy, Expressing, Incubation, Staining, Labeling, Control, Derivative Assay

Journal: World Journal of Gastroenterology

Article Title: Evaluation of a 3,5-diethoxycarbonyl-1,4-dihydrocollidine diet-induced mouse model in a comparative experimental study of portal hypertension

doi: 10.3748/wjg.v32.i9.114207

Figure Lengend Snippet: The 3,5-diethoxycarbonyl-1,4-dihydrocollidine diet mouse model (4 weeks) exhibited moderate biliary fibrosis in the portal vein region. A: Hematoxylin-eosin, Masson, and Sirius red staining of mouse livers; B-G: Statistical analysis of Masson and Sirius red staining results; H: Immunohistochemistry staining for collagen-1, α-smooth muscle actin (SMA), and desmin in mouse livers; I-Q: Statistical analysis of collagen-1, α-SMA, and desmin staining results. Sample sizes ( n ): 8:8 (normal chow diet vs 3,5-diethoxycarbonyl-1,4-dihydrocollidine), 8:7 (sham vs bile duct ligation), and 8:7:8 (oil vs 8-week carbon tetrachloride vs 12-week carbon tetrachloride). a P < 0.05. b P < 0.01. c P < 0.001. NS: No significant; NCD: Normal chow diet; DDC: 3,5-diethoxycarbonyl-1,4-dihydrocollidine; BDL: Bile duct ligation; CCl 4 : Carbon tetrachloride; HE: Hematoxylin-eosin; COL1: Collagen-1; SMA: Smooth muscle actin.

Article Snippet: Primary antibodies against collagen 1 (1:2500, catalog No. 67288-1-Ig, Proteintech), α-smooth muscle actin (SMA) (1:200, catalog No. ab5694, Abcam), desmin (1:4000, catalog No. 16520-1-AP, Proteintech), lymphatic vessel endothelial hyaluronan receptor 1 (LyVE-1) (1:100, catalog No. ab219556, Abcam), cluster of differentiation (CD) 34 (1:1000, catalog No. 14486-1-AP, Proteintech), von Willebrand factor (vWF) (1:200, catalog No. 27186-1-AP, Proteintech), vascular endothelial growth factor receptor 2 (VEGFR2) (1:150, catalog No. ab2349, Abcam), vascular endothelial growth factor A (VEGF-A) (1:100, catalog No. ab52917, Abcam), and CD31 (1:5000, catalog No. 11265-1-AP, Proteintech) were applied overnight at 4 °C, followed by 60-minute incubation with secondary antibodies at room temperature.

Techniques: Staining, Immunohistochemistry, Ligation